Research and Development - Biosolids

Title: The Detection of Astrovirus in Sludge Biosolids using an Integrated Cell Culture Nested PCR Technique.

Authors: C. D. Chapron *, N. A. Ballester and A. B. Margolin
Department of Microbiology, University of New Hampshire, Durham, NH

Abstract:
The work presented here demonstrates the utility of the integrated cell culture-reverse transcriptase -polymerase chain reaction (ICC-RT-PCR) coupled with nested PCR to detect human astroviruses and enteroviruses in sludge biosolids. Viruses were concentrated by beef extract elution and organic flocculation prior to analysis by a plaque assay and ICC-RT-PCR. Astroviruses were detected in all but one sample and all of the samples were positive for enteroviruses. We have demonstrated the prevalence and frequency of astrovirus in sludge as well as validate the ICC-RT-PCR/Nested PCR technique as a most useful tool when detecting viruses in sludge.

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