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| Microbial Identification | |||
| • | Introduction | ||
| • | Capabilities | ||
Microbial Identification Introduction
Analytical Services, Inc.’s Microbiology Laboratory provides microbial identification and other specialized analyses to clients worldwide. We identify microorganisms by cellular fatty acid (CFA) analysis, metabolic characterization, comparative electrophoresis and/or ribotyping. Cellular Fatty Acid (CFA) analysis is performed using the MIDI/Sherlock system, which uses gas chromatography to determine the fatty acid profile of an isolate and statistical pattern recognition software to compare it to the database. Metabolic Characterization may involve the Biolog system, Crystal ID System, MicroID, and/or API, all of which are based on an isolate’s substate utilization, fermentation or respiratory capabilities. Comparative Electrophoresis requires a presumptive identification of your unknown. This process involves an electrophoretic comparison of your isolate’s characteristics (e. g. proteins) to those of known strain(s). Ribotyping - The DuPont RiboPrint TM system generates the isolate’s genetic fingerprint from the ribosomal RNA (rRNA) operons and adjacent regions of the bacterial genome. The genetic pattern is compared to DuPont’s database for identification. Library Generation – We have Library Generation capabilities which allow us to create a unique database for your isolates. This may allow identification of specialized isolates which are not in any standard database. Strain Tracking – Comparison of isolates (strain tracking) is done using cluster analysis software which generates dendograms and 2-D principle component plots. These plots graphically depict the taxonomic relationships among isolates and are useful for comparing large numbers of isolates or for tracking strains over time. |
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P.O. Box 515
(mailing) Copyright © 2006 |
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