USEPA Methods 1623/1623.1

(Detection and enumeration of Cryptosporidium and Giardia) 

Analytical Services, Inc. (ASI) has provided EPA Method 1623 analyses for more than 300 clients since 1999 and has been providing Long-Term 2 Enhanced Surface Water Treatment Rule (LT2)-compliant data since 2001. ASI has substantial analytical capacity, and has processed more than 400 samples per month by Method 1623.

ASI is an USEPA-approved laboratory for Cryptosporidium enumeration under the LT2 Laboratory Quality Assurance Program.  In addition to Cryptosporidium, we are also approved under the EPA’s LT2 Laboratory Quality Assurance Program for the quantitative analysis of E. coli in non-potable water, including source waters, by Colilert/Quanti-Tray™.  As such, we are authorized to enter data in the EPA’s Data Collection & Tracking System (DCTS) for both Cryptosporidium and E. coli. 

ASI is also accredited under NELAP by the Florida Dept. of Health for Method 1623 in potable and non-potable water (in addition to other analytes).  ASI also holds certifications from the Departments of Health inVermont,Connecticut, andNew Mexico.

EPA Method 1623.1 - Method 1623.1 (EPA 816-R-12-001, Jan. 2012) is a recent and relatively minor modification to Method 1623, which is intended to aid recovery of protozoa from challenging water matrices.  Method 1623.1 incorporates a sodium hexametaphospate rinse into the elution step and an extra pellet wash into the immunomagnetic separation procedure (in addition to other administrative changes); sample collection and submission protocols remain unchanged.  

For more information, please click HERE for ASI’s technical document regarding EPA Method 1623 or contact our Clients Services team.